Mouse Semaphorin 4A Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untrasfected cells.
  • Source
    Monoclonal Rat IgG1 Clone # 757129
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A
    Thr33-Trp638
    Accession # Q62178
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Delgoffe, G. et al. (2013) Nature 501:252.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A Monoclonal Antibody (Catalog # MAB8166) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4A
Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.
  • References:
    1. Delgoffe, G.M. et al (2013) Nature 501:252.
  • Entrez Gene IDs:
    64218 (Human); 20351 (Mouse); 310630 (Rat)
  • Alternate Names:
    CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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