Key Product Details

Species Reactivity

Mouse

Applications

Neutralization, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 757129
View all Semaphorin 4A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A
Thr33-Trp638
Accession # Q62178

Specificity

Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untrasfected cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Semaphorin 4A Antibody

Detection of Semaphorin 4A antibody in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4A and eGFP antibody by Flow Cytometry.

Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A Monoclonal Antibody (Catalog # MAB8166) or (B) Rat IgG1Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse Semaphorin 4A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP

Neutralization

Delgoffe, G. et al. (2013) Nature 501:252.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.

References

  1. Delgoffe, G.M. et al (2013) Nature 501:252.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Q62178

Additional Semaphorin 4A Products

Product Documents for Mouse Semaphorin 4A Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Semaphorin 4A Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Semaphorin 4A Antibody

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