Mouse Siglec-G Alexa Fluor® 350-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7775U-100UG
R&D Systems Antibodies
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Mouse Siglec-G Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec-G in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.
Source
Monoclonal Rat IgG2a Clone # 805903
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
Glu20-Gln525
Accession # NP_766488
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Mouse CD11b+ splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Siglec-G

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.

Long Name
Sialic Acid Binding Ig-like Lectin G
Entrez Gene IDs
243958 (Mouse)
Alternate Names
PRO940;Sialic acid-binding Ig-like lectin 10;SIGLEC10;SIGLEC-10;SLG2; Siglec-10; SiglecG; Siglec-G

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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