Mouse Siglec-G Antibody

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Product Details
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Mouse Siglec-G Antibody Summary

Species Reactivity
Detects mouse Siglec-G in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.
Monoclonal Rat IgG2A Clone # 805903
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
Accession # NP_766488
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
Mouse CD11b+ splenocytes
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-G

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.

Long Name
Sialic Acid Binding Ig-like Lectin G
Entrez Gene IDs
243958 (Mouse)
Alternate Names
PRO940;Sialic acid-binding Ig-like lectin 10;SIGLEC10;SIGLEC-10;SLG2; Siglec-10; SiglecG; Siglec-G

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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