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Mouse Siglec-H APC-conjugated Antibody

Catalog #: FAB7319A Datasheet / COA / SDS

Discontinued Product

FAB7319A has been discontinued.
View all Siglec-H products.
Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry.
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Mouse Siglec-H APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec-H.
Source
Monoclonal Rat IgG1 Clone # 730407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-H
Met1-Gly269
Accession # NP_848821
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec‑H antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Siglec-H APC-conjugated Monoclonal Antibody (Catalog # FAB7319A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-H

Siglec-H, a murine CD33-related Siglec-like molecule and a member of the Sialic Acid-binding Immunoglobulin (Ig)-like Lectin (Siglec) family, is expressed specifically on Plasmacytoid Dendritic Cell (pDC) precursors in the bone marrow, spleen, blood, and lymph nodes.

Long Name
Sialic Acid Binding Ig-like Lectin 7
Entrez Gene IDs
233274 (Mouse)
Alternate Names
6430529G09Rik; Siglech sialic acid binding Ig-like lectin H; SiglecH; Siglec-H

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COA
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