Mouse TCF7/TCF1 PE-conjugated Antibody

IC8224P has been discontinued. View all TCF7/TCF1 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects mouse TCF7/TCF1 in direct ELISAs and Western Blots.
  • Source
    Monoclonal Rat IgG2B Clone # 812145
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TCF7/TCF1
    Accession # Q00417
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 PE‑conjugated Monoclonal Antibody (Catalog # IC8224P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TCF7/TCF1
TCF7 (Transcription Factor 7), also known as TCF1 (T cell Factor 1) is a member of the Lymphoid Enhancer Binding Factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within amino acids (aa) 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.
  • Long Name:
    Transcription Factor 7
  • Entrez Gene IDs:
    6932 (Human); 21414 (Mouse); 363595 (Rat)
  • Alternate Names:
    FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Mouse TCF7/TCF1 PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order


Customer Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Panels For

Learn More
Monoclonal Antibodies
Associated Pathways
R&D Systems Guarantee