Mouse TCF7/TCF1 PE‑conjugated Antibody

R&D Systems | Catalog # IC8224P

R&D Systems
Discontinued Product
IC8224P has been discontinued. View all TCF7/TCF1 products.

Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 812145
View all TCF7/TCF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TCF7/TCF1
Gly61-Gly116
Accession # Q00417

Specificity

Detects mouse TCF7/TCF1 in direct ELISAs and Western Blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TCF7/TCF1 PE‑conjugated Antibody

Detection of TCF7/TCF1 antibody in EL‑4 Mouse Cell Line antibody by Flow Cytometry.

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry.

EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 PE-conjugated Monoclonal Antibody (Catalog # IC8224P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse TCF7/TCF1 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: EL‑4 mouse lymphoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCF7/TCF1

TCF7 (Transcription Factor 7), also known as TCF1 (T cell Factor 1) is a member of the Lymphoid Enhancer Binding Factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within amino acids (aa) 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name

Transcription Factor 7

Alternate Names

TCF1

Entrez Gene IDs

6932 (Human); 21414 (Mouse); 363595 (Rat)

Gene Symbol

TCF7

UniProt

Q00417

Additional TCF7/TCF1 Products

Product Documents for Mouse TCF7/TCF1 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TCF7/TCF1 PE‑conjugated Antibody

For research use only

Customer Reviews for Mouse TCF7/TCF1 PE‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse TCF7/TCF1 PE‑conjugated Antibody and earn rewards!

Have you used Mouse TCF7/TCF1 PE‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies