Mouse TCR gamma/delta Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TCR gamma /δ.
  • Source
    Monoclonal Hamster IgG2, kappa Clone # GL-3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse intraepithelial lymphocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TCR  gamma /δ in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR  gamma /δ Monoclonal Antibody (Catalog # MAB7297) or (B) Normal Hamster IgG Control Antibody followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody. 
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCR gamma/delta
The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).
  • References:
    1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.
  • Entrez Gene IDs:
    915 (Human); 12500 (Mouse); 25710 (Rat)
  • Alternate Names:
    CD3d; delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCRG; TiT3; TRG
Related Research Areas
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