Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma /δ Monoclonal Antibody (Catalog # MAB7297) or (B) Normal Hamster IgG Control Antibody followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCR gamma/delta
The gamma δ
T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma
and CD3δ glycoprotein subunits of the Ig
superfamily. T gamma δ cells develop as a minor
population in the thymus and migrate mainly to in skin and intestinal
epithelial layers. Mouse and rat CD3 gamma and
CD3δ are synthesized as 182 and 173 amino
acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and
84 aa extracellular domains (ECD), respectively. The germline ECD sequences of
CD3 gamma and CD3δ
share 71% and 76% aa identity between mouse and rat, respectively, while both
proteins and species share 57-62% aa identity with human CD3 gamma
and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).
Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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