Mouse TER-119 Fluorescein-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER‑119 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 degreesC as supplied.
The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).
- Kina, T. et al. (2000) Br. J. Haematol. 109:280.
- Ikuta, K. et al. (1990) Cell 62:863.
- Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
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