Rat IgG2B Fluorescein-conjugated Antibody

  (3 citations)   
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
  • Specificity
    Selected for use as a rat IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2B Clone # 141945
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry Mouse thymocytes werestained with Rat Anti-Mouse CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog #FAB2160F,filled histogram) or Rat IgG Fluorescein-conjugatedIsotype Control Antibody (Catalog # IC013F, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib.
    Authors: Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I, Nakamura Y
    BMC Cancer, 2015;15(0):260.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013;190(7):3696-705.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007;37(2):456-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl

FAQs

No product specific FAQs exist for this product, however you may

View all Isotype Control FAQs
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 700-conjugated Antibody

Ctrl IC013N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B PerCP-conjugated Isotype Control

Ctrl IC013C
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC013S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC013R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Biotinylated Isotype Control

Ctrl IC013B
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC013T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC013V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2B Isotype Control

Ctrl MAB0061R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Rat IgG2B Fluorescein-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies