Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 APC‑conjugated Monoclonal Antibody (Catalog # IC7640A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TLR11 is a 110-120 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor family of proteins that is primarily expressed in intestinal and bladder epithelial cells, dendritic cells, macrophages, astrocytes, neurons and microglia. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats, a 21 aa transmembrane (TM) domain, and a 184 aa cytoplasmic region with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER) where it heterodimerizes with TLR12, and interacts with the 12-TM multispan ER protein UNC93B1, that serves as chaperone and co-factor for TLR11 activity. The receptor recognizes flagelin and profilin from Toxoplasma gondii, plus components of E.coli, S.typhimurium, and promotes IL-12 production by dendritic cells.
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