Mouse TLR11 APC‑conjugated Antibody

R&D Systems | Catalog # IC7640A

R&D Systems
Discontinued Product
IC7640A has been discontinued. View all TLR11 products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 786404
View all TLR11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TLR11
Thr209-Gln325
Accession # Q6R5P0

Specificity

Detects mouse TLR11 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TLR11 APC‑conjugated Antibody

Detection of TLR11 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 APC-conjugated Monoclonal Antibody (Catalog # IC7640A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse TLR11 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TLR11

TLR11 is a 110-120 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor family of proteins that is primarily expressed in intestinal and bladder epithelial cells, dendritic cells, macrophages, astrocytes, neurons and microglia. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats, a 21 aa transmembrane (TM) domain, and a 184 aa cytoplasmic region with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER) where it heterodimerizes with TLR12, and interacts with the 12-TM multispan ER protein UNC93B1, that serves as chaperone and co-factor for TLR11 activity. The receptor recognizes flagelin and profilin from Toxoplasma gondii, plus components of E.coli, S.typhimurium, and promotes IL-12 production by dendritic cells.

Long Name

Toll-like Receptor 11

Alternate Names

Gm287

Entrez Gene IDs

239081 (Mouse); 498491 (Rat)

Gene Symbol

TLR11

UniProt

Q6R5P0

Additional TLR11 Products

Product Documents for Mouse TLR11 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TLR11 APC‑conjugated Antibody

For research use only

Citations for Mouse TLR11 APC‑conjugated Antibody

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