Mouse TREML1/TLT‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF2424AFP680

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all TREML1/TLT-1 Primary Antibodies »

Product Specifications

Specificity

Detects mouse TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human TREML1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TREML1/TLT‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TREML1/TLT-1

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha ‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

References

  1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
  2. Washington, A.V. et al. (2004) Blood 104:1042.
  3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
  4. Washington, A.V. et al. (2002) Blood 100:3822.
  5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Alternate Names

TLT-1, TLT1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Gene Symbol

TREML1

UniProt

NP_082039

Additional TREML1/TLT-1 Products

Product Documents for Mouse TREML1/TLT‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TREML1/TLT‑1 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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