Mouse TRIM5 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3550T Datasheet / COA / SDS

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AF3550T-100UG

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Mouse TRIM5 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TRIM5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse TRIM5
Ala2-Gln249
Accession # AAH38937

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as homotrimers. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Mouse TRIM5 shares 49% and 81% amino acid sequence homology with human and rat TRIM5, respectively.

Long Name

Tripartite Motif-containing 5

Entrez Gene IDs

85363 (Human)

Alternate Names

EC 6.3.2; EC 6.3.2.-; RING finger protein 88; RNF88; RNF88tripartite motif protein TRIM; TRIM5; TRIM5alpha; tripartite motif containing 5; tripartite motif protein TRIM5; tripartite motif-containing 5; tripartite motif-containing protein 5