Mouse Tryptase  epsilon /BSSP‑4 Alexa Fluor™ Plus 647‑conjugated Antibody

Tryptase epsilon, brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing result indicates that the purified protein corresponds to the pro enzyme. After activation with thermolysin, the enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The thioester activity is inhibited by 2 mM AEBSF (R&D Systems, Catalog # EI001), a general serine protease inhibitor, and by recombinant human Serpin A5 (R&D Systems, Catalog # 1266-PI).