UGRP1 (also secretglobin gene family 3A2/SCGB3A2) is an 18 kDa, secreted nonglycosylated polypeptide that is a member of the Uteroglonin family, UGRP subfamily. Mouse UGRP1(isoform A) circulates as a 37 kDa homodimer. Its mature form is 70 amino acids in length. There are two large alternate splice forms termed B and C. UGRP1B is a secreted homodimer that shows a 22 aa insert between aa 84 and 85 of the short form. UGRP1C contains a 55 aa substitution for the C-terminal seven aa of the short form, predicting a 118 aa secreted form. The common portion of mature mouse UGRP1 (isoforms A, B and C) shares 86% and 81% aa identity with rat and human UGRP1, respectively.
Mouse UGRP1/SCGB3A2 Antibody
R&D Systems | Catalog # MAB3465
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Transgenic Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 381707
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Product Specifications
Immunogen
E. coli-derived recombinant mouse UGRP1/SCGB3A2 isoform C
Leu22-Leu139
Accession # Q920H1
Leu22-Leu139
Accession # Q920H1
Specificity
Detects mouse UGRP1/SCGB3A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UGRP1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse UGRP1/SCGB3A2 Antibody
Detection of Mouse UGRP1/SCGB3A2 by Immunohistochemistry
Basal cell (BC) subpopulations other than BC-1 and BC-2 are relatively rare and have no consistent spatial distribution in the adult tracheal epithelium.Representative tile scan pictures of adult mouse tracheal sections immunostained for KRT5, TRP63 and representative markers of Basal-proliferating (A), Basal-squamous (B), Basal-mesenchyme-like (C), and Basal-secretory (D). High magnification images are representative of specific BC subpopulations depicted in boxed areas along the anterior-posterior or dorsal-ventral axis of the tracheal epithelium. Scale bars: large panels of overview: 200 μm; small panels: 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36178196), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse UGRP1/SCGB3A2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse UGRP1/SCGB3A2
Sample: Recombinant Mouse UGRP1/SCGB3A2
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: UGRP1/SCGB3A2
Long Name
Uteroglobin-related Protein 1
Alternate Names
PnSP-1, SCGB3A2
Gene Symbol
SCGB3A2
UniProt
Additional UGRP1/SCGB3A2 Products
Product Documents for Mouse UGRP1/SCGB3A2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse UGRP1/SCGB3A2 Antibody
For research use only
Related Research Areas
Citations for Mouse UGRP1/SCGB3A2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars