Mouse WIF-1 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB135V-100UG

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Mouse WIF-1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human WIF-1 is observed.
Source
Monoclonal Rat IgG2a Clone # 134714
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse WIF-1
Gly29-Trp379
Accession # Q9WUA1
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WIF-1

WIF-1 is a secreted protein of approximately 50 kDa that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Mature mouse WIF-1 shares 95% and 98% amino acid sequence identity with human and rat WIF-1, respectively.

Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

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