MSPR/Ron Antibody (SD2006)

Novus Biologicals | Catalog # NBP2-67814

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD2006 expressed in HEK293
View all MSPR/Ron Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MSPR/Ron aa 11-60 / 1,400. (SwissProt: Q04912 Human)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MSPR/Ron Antibody (SD2006)

Western Blot: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Western Blot: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Western Blot: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of RON on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of paraffin-embedded human lung cancer tissue using anti-RON antibody. Counter stained with hematoxylin.
Flow Cytometry: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Flow Cytometry: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Flow Cytometry: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of THP-1 cells with RON antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/ Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814]

Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-RON antibody. Counter stained with hematoxylin.

Applications for MSPR/Ron Antibody (SD2006)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSPR/Ron

Ron, the tyrosine kinase receptor for the Macrophage-stimulating protein, is involved in cell dissociation, motility, and matrix invasion (1). Ron, a cDNA homologous to the hepatocyte growth factor (HGF) receptor gene (MET), encodes a putative tyrosine kinase. It has been shown that the Ron gene is expressed in several epithelial tissues as well as in granulocytes and monocytes. The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface (2).

Long Name

Macrophage Stimulating Protein Receptor

Alternate Names

CD136, MSP R, MST1R, Ron

Gene Symbol

MST1R

Additional MSPR/Ron Products

Product Documents for MSPR/Ron Antibody (SD2006)

Certificate of Analysis

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Product Specific Notices for MSPR/Ron Antibody (SD2006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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