MST2/STK3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19421

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MST2/STK3. The exact sequence is proprietary.

Localization

Cytoplasm (By similarity); Nucleus (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST2/STK3 Antibody - BSA Free

Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421] - STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody diluted by 1:1000.
Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2 Antibody [NBP2-19421] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2 Antibody [NBP2-19421] - Sample (30 ug of whole cell lysate) A: THP-1 B: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421]

Western Blot: MST2/STK3 Antibody [NBP2-19421] - STK3 antibody immunoprecipitates STK3 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of STK3 protein by 4 ug STK3 antibody 7.5 % SDS-PAGE.The immunoprecipitated STK3 protein was detected by STK3 antibody diluted at 1:500. Anti-rabbit IgG was used as a secondary reagent

Applications for MST2/STK3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

UniProt

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MST2/STK3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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