Recombinant Human MTHFS His Protein

Name: Recombinant Human MTHFS His Protein
Brand: Novus Biologicals
SKU: NBP1-51046
Price: 929 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-51046

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all MTHFS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human MTHFS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MTHFS His Protein

SDS-PAGE: Recombinant Human MTHFS His Protein [NBP1-51046]

SDS-Page: Recombinant Human MTHFS Protein [NBP1-51046] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-51046

Formulation	20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 30% glycerol, 0.2 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTHFS

MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD). Recombinant human MTHFS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase), 5-formyltetrahydrofolate cyclo-ligase, EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase, FLJ30410, HsT19268, Methenyl-THF synthetase

Gene Symbol

MTHFS

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Product Documents for Recombinant Human MTHFS His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human MTHFS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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