MUC5B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92151

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MUC5B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CTCTYEDRTYSYQDVIYNTTDGLGACLIAICGSNGTIIRKAVACPGTPATTPFTFTTAWVPHSTTSPALPVSTVCVREVCRWSSWYNGHRPEPGLGGGDFETFENLRQRGYQVCPVLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MUC5B Antibody - BSA Free

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Analysis in human cervix, uterine and skeletal muscle tissues. Corresponding MUC5B RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MUC5B Antibody [NBP1-92151]

Immunocytochemistry/ Immunofluorescence: MUC5B Antibody [NBP1-92151]

Immunocytochemistry/Immunofluorescence: MUC5B Antibody [NBP1-92151] - Staining of human cell line A549 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human gallbladder shows very strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151]

Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human lower gastrointestinal shows strong extracellular space positivity in glandular cells.
MUC5B Antibody - BSA Free Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
MUC5B Antibody - BSA Free Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Staining of human cervix, uterine shows very strong extracellular space positivity in glandular cells.
MUC5B Antibody - BSA Free Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Immunohistochemistry: MUC5B Antibody - BSA Free [NBP1-92151]

Analysis in human cervix, uterine and skeletal muscle tissues using NBP1-92151 antibody. Corresponding MUC5B RNA-seq data are presented for the same tissues.

Applications for MUC5B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MUC5B

MUC5B, also known as Mucin 5B, is a long 5762 amino acid protein that is 596 kDa, expressed on surface airway epithelia, it is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. Disease research is being performed in relation to MUC5B and chronic obstructive pulmonary disease, cervicitis, sinusitis, polyposis, pseudomyxoma peritonei, otitis media, dry eye syndrome, ampulla of vater carcinoma, common cold, copd, dental caries, diffuse panbronchiolitis, biliary papillomatosis, panbronchiolitis, atrophic gastritis, Barrett's esophagus, cystic fibrosis lung disease, peptic ulcer, bronchitis, gastritis, sinusitis, asthma, laryngitis, and nasopharyngitis. This protein has been shown to have interactions with AMY1A, AMY1B, AMY1C, HTN1, STATH, and over 40 other proteins in Addition of GalNAc to the Tn antigen via an alpha-1,6 linkage forms a Core 7 glycoprotein, O-linked glycosylation of mucins, Metabolism of proteins, Post-translational protein modification, Addition of galactose to the Tn antigen via an alpha-1,3 linkage forms a Core 8 glycoprotein, Addition of GlcNAc to the Tn antigen via a beta-1,6 linkage forms a Core 6 glycoprotein, Termination of O-glycan biosynthesis, Salivary secretion, Mucin expression in CF via TLRs, EGFR signaling pathways, Mucin expression in CF via IL-6, IL-17 signaling pathways, and Selected targets of CREB1 pathways.

Alternate Names

high molecular weight salivary mucin MG1, mucin 5, subtype B, tracheobronchial, mucin 5B, oligomeric mucus/gel-forming, sublingual gland mucin, tracheobronchial

Gene Symbol

MUC5B

Additional MUC5B Products

Product Documents for MUC5B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MUC5B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MUC5B Antibody - BSA Free

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Protocols

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FAQs

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