Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66710

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Urine, Cell Supernatant, Tissue Homogenate (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31-20 ng/mL
View all Muscle Phosphofructokinase/PFKM ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Rat

Specificity

This kit recognizes Rat 6-PFK in samples. No significant cross-reactivity or interference between Rat 6-PFK and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 9.25%

Inter-Assay Precision (Precision between assays) CV% 10.9%

Recovery

Recovery

88-112%

Linearity

Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence)

Scientific Data Images

Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence)

ELISA: Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence) [NBP2-66710] -

ELISA: Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence) [NBP2-66710] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Muscle Phosphofructokinase/PFKM

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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