Mycobacterium avium subsp. paratuberculosis Antibody (6G11) - BSA Free

Novus Biologicals | Catalog # NBP2-54646

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 6G11

Format

BSA Free
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Product Specifications

Immunogen

Gamma-irradiated whole MAP strain B4 cells

Reactivity Notes

Mycobacterium avium

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Description

The clone number is listed as Z5P2D9*A9 in the scientific literature.

Applications

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunoprecipitation

1:10 - 1:500
Application Notes
Positive Control: Mycobacterium avium subsp. paratuberculosis whole cells

Formulation, Preparation, and Storage

Purification

Thiophilic Chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mycobacterium avium subsp. paratuberculosis

Bacterial suspension of irradiated MAP was added wells as 'free MAP cells' prior to the addition of the antibody. Negative control wells received assay buffer followed by the antibody. A decrease in absorbance values in the presence of 'free MAP cells' over negative control wells is indicative of MAP binding. Figure depicts the mean absorbance values (n = 3) standard error. Mean % binding inhibition was calculated based on the difference in the%binding of the antibody to the attached MAP cells in the presence and absence of 'free MAP cells', where negative control wells represent 100% binding.

Alternate Names

Mycobacterium paratuberculosis

Additional Mycobacterium avium subsp. paratuberculosis Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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