Myelin expression factor 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12682

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Myelin expression factor 2 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for Myelin expression factor 2 Antibody - BSA Free

Western Blot: Myelin expression factor 2 Antibody [NBP3-12682]

Western Blot: Myelin expression factor 2 Antibody [NBP3-12682]

Western Blot: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A: SH-SY5Y Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:70 kDa
Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution.
Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human testis with rabbit polyclonal antibody at 1:10000 dilution.
Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]

Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human brain with rabbit polyclonal antibody at 1:10000 dilution.
Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682]

Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682]

Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 64 kDaObserved band size :64 kDa

Applications for Myelin expression factor 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:1000-1:20000

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myelin expression factor 2

Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA

Alternate Names

FLJ11213, HsT18564, KIAA1341MSTP156, MEF-2myEF-2, MGC87325, MST156, MyEF-2, myelin expression factor 2, myelin gene expression factor 2

Gene Symbol

MYEF2

Additional Myelin expression factor 2 Products

Product Documents for Myelin expression factor 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myelin expression factor 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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