Key Product Details

Species Reactivity

Human

Applications

Immunoassay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E2cc

Format

BSA Free
View all Myoglobin Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human cardiac myoglobin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Myoglobin Antibody (4E2cc) - BSA Free (NB600-1291) is a monoclonal antibody validated for use in Immunoassay,Sandwich ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Myoglobin Antibody (4E2cc) - BSA Free

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000
Application Notes
Human cardiac myoglobin immunoassay. Recommended pairs for sandwich immunoassay (capture-detection): NB600-1291 - NB120-8345, NB120-8345 - NB600-1291

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Myoglobin

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Alternate Names

MB

Entrez Gene IDs

4151 (Human)

Gene Symbol

MB

Additional Myoglobin Products

Product Documents for Myoglobin Antibody (4E2cc) - BSA Free

Certificate of Analysis

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Product Specific Notices for Myoglobin Antibody (4E2cc) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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