MYSM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-17396

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MYSM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against recombinant protein corresponding to amino acids: HYLDSSWRTENGLIPWTLDNTISEENRAVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYSM1 Antibody - BSA Free

Western Blot: MYSM1 Antibody [NBP3-17396]

Western Blot: MYSM1 Antibody [NBP3-17396]

Western Blot: MYSM1 Antibody [NBP3-17396] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: MYSM1 Antibody [NBP3-17396]

Immunocytochemistry/ Immunofluorescence: MYSM1 Antibody [NBP3-17396]

Immunocytochemistry/Immunofluorescence: MYSM1 Antibody [NBP3-17396] - Staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.
MYSM1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MYSM1 Antibody - BSA Free [NBP3-17396]

Chromatin Immunoprecipitation-exo-Seq: MYSM1 Antibody - BSA Free [NBP3-17396]

ChIP-Exo-Seq composite graph for Anti-MYSM1 (NBP3-17396) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for MYSM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYSM1

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.

Long Name

Myb-Like, SWIRM And MPN Domains 1

Alternate Names

2A-DUB, Histone H2A Deubiquitinase MYSM1

Gene Symbol

MYSM1

Additional MYSM1 Products

Product Documents for MYSM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MYSM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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