NADA-green
Tocris Bioscience | Catalog # 6648
Key Product Details
Description
Wavelength
Product Description
NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.Please note that the spectral data is solvent dependant
Optical Data
| Emission Color | Green |
| λabs | 450 nm |
| λem | 555 nm |
| Extinction Coefficient (ε) | 25,000 M-1cm-1 |
| Application | Fluorescence Microscopy |
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Product Specifications for NADA-green
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.37 | 100 |
Preparing Stock Solutions for NADA-green
The following data is based on the product molecular weight 303.66.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.29 mL | 16.47 mL | 32.93 mL |
| 5 mM | 0.66 mL | 3.29 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.29 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 7 publications citing the usage of this product.
- Kuru Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 2014 PMID: 25474031
- Ladokhin Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 2002 PMID: 12427023
- Kuru In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 2012 PMID: 23055266
Product Documents for NADA-green
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NADA-green
For research use only
Citations for NADA-green
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