SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

Novus Biologicals | Catalog # NBP3-52466

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3063
View all SLC6A2/NET Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P23975, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of human locus coeruleus shows strong cytoplasmic positivity in noradrenaline neurons.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of mouse brain shows strong positivity in noradrenergic fibers in the cerebral cortex.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of mouse brain shows strong positivity in noradrenergic fibers in the hippocampus.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of rat cerebellum shows strong positivity in noradrenergic fibers.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) [NBP3-52466]

Staining of rat pons shows strong positivity in noradrenaline neurons in the locus coeruleus.

Applications for SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC6A2/NET

Norepinephrine Transporter [NET] (or noradrenaline transporter (NAT)) is a monoamine transporter that transports the neurotransmitter noradrenaline from the synapse back to its vesicles for storage until later use. It also appears to transport the neurotransmitter dopamine in the same way, but to a lesser degree. Studies have shown a decrease in NET levels in the locus coeruleus in patients diagnosed with major depression (Klimek et al., 1997). Cocaine, amphetamines and many therapeutic antidepressants, such as the SNRIs (Serotonin-norepinephrine reuptake inhibitors) and the tricyclic antidepressants (TCAs) act to raise noradrenaline. Furthermore, deficits in the NET gene have been associated with ADHD (Kim et al., 2006).

Long Name

Norepinephrine Transporter

Alternate Names

NAT1, Noradrenalin Transporter, Norepinephrine Transporter

Gene Symbol

NET1, SLC6A2

Additional SLC6A2/NET Products

Product Documents for SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

Certificate of Analysis

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Product Specific Notices for SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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