NAI

Catalog # Availability Size / Price Qty
7004/10
NAI | CAS No. 1055970-47-2 | DNA, RNA and Protein Synthesis Compounds
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Description: Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable

Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone

Product Details
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Biological Activity

NAI is a reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data

M.Wt:
187.2
Formula:
C10H9N3O
Solubility:
Soluble to 100 mM in DMSO
Storage:
Store at -20°C
CAS No:
1055970-47-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Comparison of SHAPE reagents for mapping RNA structures inside living cells.
    Lee et al.
    RNA, 2017;23:169
  2. Determination of in vivo RNA structure in low-abundance transcripts.
    Kwok et al.
    Nat.Commun., 2013;4:2971
  3. RNA SHAPE analysis in living cells.
    Spitale et al.
    Nat.Chem.Biol., 2013;9:18

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