NARS2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82077

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NARS2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAEFYMIEAEISFVDSLQDLMQVIEELFKATTMMVLSKCPEDVELCHKFIAPGQKDRLEHMLKNNFLIISYTEAVEILKQASQNFTFTPEWGADLRTEHEKYLVKHCGNIPVFVINYPLTLKPFYMRDNEDGPQHTVAAVDLLVPGVG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NARS2 Antibody - BSA Free

Western Blot: NARS2 Antibody [NBP1-82077]

Western Blot: NARS2 Antibody [NBP1-82077]

Western Blot: NARS2 Antibody [NBP1-82077] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: NARS2 Antibody [NBP1-82077]

Immunocytochemistry/ Immunofluorescence: NARS2 Antibody [NBP1-82077]

Immunocytochemistry/Immunofluorescence: NARS2 Antibody [NBP1-82077] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NARS2 Antibody [NBP1-82077]

Immunohistochemistry-Paraffin: NARS2 Antibody [NBP1-82077]

Immunohistochemistry-Paraffin: NARS2 Antibody [NBP1-82077] - Staining of human kidney shows strong cytoplasmic positivity with a granular pattern in tubule cells.
Simple Western: NARS2 Antibody [NBP1-82077]

Simple Western: NARS2 Antibody [NBP1-82077]

Simple Western: NARS2 Antibody [NBP1-82077] - Simple Western lane view shows a specific band for NARS2 in 0.5 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: NARS2 Antibody [NBP1-82077]

Simple Western: NARS2 Antibody [NBP1-82077]

Simple Western: NARS2 Antibody [NBP1-82077] - Electropherogram image(s) of corresponding Simple Western lane view. NARS2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Applications for NARS2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 54 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 4 using NBP1-82077 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NARS2

Alternate Names

AsnRS, asparagine tRNA ligase 2, mitochondrial (putative), Asparagine--tRNA ligase, asparaginyl-tRNA synthetase 2, mitochondrial (putative), EC 6.1.1, EC 6.1.1.22, FLJ23441, probable asparaginyl-tRNA synthetase, mitochondrial, SLM5

Gene Symbol

NARS2

Additional NARS2 Products

Product Documents for NARS2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NARS2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NARS2 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used NARS2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human fibroblast and HeLa whole cell lysate
    Species: Human
    Verified Customer | Posted 08/13/2014
    NARS2 (AsnRS) in human fibroblast lysate. Insert shows the 50 kDa molecular weight marker on the same membrane.
    NARS2 Antibody - BSA Free NBP1-82077

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies