NCAPH Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88346

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCAPH Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEMTDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNESVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSLGDDFDANDEPDHT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCAPH Antibody - BSA Free

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88346 (A) shows similar protein distribution across tissues to independent antibody NBP1-88345 (B).
Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining in human testis and kidney tissues. Corresponding NCAPH RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human kidney shows no positivity as expected.
Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human skin shows nuclear positivity in epidermal cells.
Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human tonsil shows nuclear positivity in germinal center cells.
NCAPH Antibody - BSA Free Western Blot: NCAPH Antibody - BSA Free [NBP1-88346]

Western Blot: NCAPH Antibody - BSA Free [NBP1-88346]

Analysis in human cell lines U2OS and SK-MEL-30 using Anti-NCAPH antibody. Corresponding NCAPH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for NCAPH Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCAPH

NCAPH encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.

Alternate Names

barren (Drosophila) homolog, barren homolog (Drosophila), barren homolog 1, barren homolog 1 (Drosophila), Barren homolog protein 1, BRRN, BRRN1CAP-H, CAPH, Chromosome-associated protein H, condensin complex subunit 2, HCAP-H, KIAA0074, Non-SMC condensin I complex subunit H, non-SMC condensin I complex, subunit H, XCAP-H homolog

Gene Symbol

NCAPH

Additional NCAPH Products

Product Documents for NCAPH Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCAPH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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