NCOA4 Antibody (1E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00008031-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E5

Format

Azide and BSA Free
View all NCOA4 Primary Antibodies »

Product Specifications

Immunogen

NCOA4 (NP_005428, 505 a.a. ~ 614 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQM

Specificity

NCOA4 (1E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NCOA4 Antibody (1E5) - Azide and BSA Free

Western Blot: NCOA4 Antibody (1E5) [H00008031-M03]

Western Blot: NCOA4 Antibody (1E5) [H00008031-M03]

Western Blot: NCOA4 Antibody (1E5) [H00008031-M03] - Analysis of NCOA4 expression in Hela S3 NE (Cat # L013V3).
Immunohistochemistry-Paraffin: NCOA4 Antibody (1E5) [H00008031-M03]

Immunohistochemistry-Paraffin: NCOA4 Antibody (1E5) [H00008031-M03]

Immunohistochemistry-Paraffin: NCOA4 Antibody (1E5) [H00008031-M03] - Analysis of monoclonal antibody to NCOA4 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml
ELISA: NCOA4 Antibody (1E5) [H00008031-M03]

ELISA: NCOA4 Antibody (1E5) [H00008031-M03]

ELISA: NCOA4 Antibody (1E5) [H00008031-M03] - Detection limit for recombinant GST tagged NCOA4 is approximately 0.3ng/ml as a capture antibody.

Applications for NCOA4 Antibody (1E5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 22887727).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCOA4

The NCOA4 gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gen

Alternate Names

Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator

Entrez Gene IDs

8031 (Human)

Gene Symbol

NCOA4

OMIM

188550 (Human)

UniProt

NP_005428 (Human)

Additional NCOA4 Products

Product Documents for NCOA4 Antibody (1E5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NCOA4 Antibody (1E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCOA4 Antibody (1E5) - Azide and BSA Free

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Protocols

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FAQs

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