NDUFS8 Antibody
Novus Biologicals | Catalog # NBP1-49847
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Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Canine (100%), Human (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-EAEIAANIQAD corresponding to internal region according to NP_002487.1.
Epitope
EAEIAANIQAD - internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NDUFS8 Antibody
Western Blot: NDUFS8 Antibody [NBP1-49847]
Western Blot: NDUFS8 Antibody [NBP1-49847] - Western blot analysis of NDUFS8 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49847 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for NDUFS8 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:4000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in mouse and rat heart lysates (calculated MW of 24.0 kDa band according to mouse NP_659119.2 and rat NP_001099792.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NDUFS8
Alternate Names
CI-23k, CI23KD, CI-23kD, complex I 23kDa subunit, Complex I-23kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 8 (23kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY, TYKY subunit
Gene Symbol
NDUFS8
UniProt
Additional NDUFS8 Products
Product Documents for NDUFS8 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NDUFS8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NDUFS8 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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