NDUFS8 Antibody

Novus Biologicals | Catalog # NBP2-26107

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NDUFS8 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AEPRADGSRR corresponding to internal region according to NP_002487.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDUFS8 Antibody

Western Blot: NDUFS8 Antibody [NBP2-26107]

Western Blot: NDUFS8 Antibody [NBP2-26107]

Western Blot: NDUFS8 Antibody [NBP2-26107] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NDUFS8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human and mouse heart lysates (calculated MW of 23.7 kDa band according to NP_002487.1). An additional fainter band of 90 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFS8

NDUFS8 (NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)) is a subunit of NADH. Mutations in NDUFS8 have been linked to Leigh syndrome.

Alternate Names

CI-23k, CI23KD, CI-23kD, complex I 23kDa subunit, Complex I-23kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 8 (23kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY, TYKY subunit

Entrez Gene IDs

4728 (Human); 225887 (Mouse); 293652 (Rat)

Gene Symbol

NDUFS8

Additional NDUFS8 Products

Product Documents for NDUFS8 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for NDUFS8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NDUFS8 Antibody

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