Necrosulfonamide

Tocris Bioscience | Catalog # 5025

Necroptosis inhibitor; also inhibits pyroptosis
Tocris Bioscience

Key Product Details

Description

Necroptosis inhibitor; also inhibits pyroptosis
View all Additional Apoptosis-related Compounds »

Product Description

Necrosulfonamide is a necroptosis inhibitor. Blocks mixed lineage kinase domain-like protein (MLKL), a critical substrate of receptor-interacting serine-threonine kinase 3 (RIP3) during necrosis. Prevents MLKL-RIP1-RIP3 necrosome complex from interacting with downstream necrosis effectors. Binds and inhibits gasdermin D. Inhibits pyroptosis.

Product Specifications for Necrosulfonamide

Molecular Weight

461.47

Formula

C18H15N5O6S2

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

(2E)-N-[4-[[(3-Methoxy-2-pyrazinyl)amino]sulfonyl]phenyl]-3-(5-nitro-2-thienyl)-2-propenamide

CAS Number

1360614-48-7

PubChem ID

1566236

InChI Key

FNPPHVLYVGMZMZ-XBXARRHUSA-N

SMILES

O=C(NC2=CC=C(S(NC3=C(OC)N=CC=N3)(=O)=O)C=C2)/C=C/C1=CC=C([N+]([O-])=O)S1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.23 20

Preparing Stock Solutions for Necrosulfonamide

The following data is based on the product molecular weight 461.47.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 10.83 mL 54.17 mL 108.35 mL
1 mM 2.17 mL 10.83 mL 21.67 mL
2 mM 1.08 mL 5.42 mL 10.83 mL
10 mM 0.22 mL 1.08 mL 2.17 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 52 publications citing the usage of this product.

Product Documents for Necrosulfonamide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Necrosulfonamide

For research use only

Citations for Necrosulfonamide

Customer Reviews for Necrosulfonamide (1)

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  • Necrosulfonamide work well to block pyroptosis
    Name: Xiaogang Wang
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: THP-1 cell line
    Verified Customer | Posted 06/12/2020
    We used Necrosulfonamide as a positive control to inhibit the induction of pyroptosis by staphylococcal cytolysin. Differentiated THP-1 cells were pretreated with 25uM Necrosulfonamide (NSA) for 1 h before treating with or without 1 ug/ml Staphylococcal pore-forming toxin for 4 h. The LDH release in culture supernatants of treated cells was measured. Our data indicated that Necrosulfonamide treatment can significantly inhibit staphylococcal cytolysin induced pyroptosis.
    Necrosulfonamide 5025

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