Nectin-2/CD112 Antibody (DMC224) - Chimeric

Novus Biologicals | Catalog # NBP3-48298

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC224
View all Nectin-2/CD112 Primary Antibodies »

Product Specifications

Immunogen

Nectin-2/CD112 recombinant protein expressed in mammalian cells (Accession# Q92692). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for Nectin-2/CD112 Antibody (DMC224) - Chimeric

Nectin-2/CD112 Antibody (DMC224) - Chimeric

Flow Cytometry: Nectin-2/CD112 Antibody (DMC224) - Chimeric [NBP3-48298]

Flow cytometry analysis of antigen binding of anti-human CD112 mAb(NBP3-48298). (A) NBP3-48298 does not bind to MM.1S cells that do not express CD112. (B) A clear peak shift of NBP3-48298 was seen compared to the control when incubated with CD112-expressing MCF-7 cells, indicating strong binding of NBP3-48298 to CD112. Antibodies were incubated at 5 ug/mL.
Nectin-2/CD112 Antibody (DMC224) - Chimeric

Flow Cytometry: Nectin-2/CD112 Antibody (DMC224) - Chimeric [NBP3-48298]

Flow cytometry analysis of antigen binding of anti-human CD112 mAb(NBP3-48298). (A) NBP3-48298 does not bind to MM.1S cells that do not express CD112. (B) A clear peak shift of NBP3-48298 was seen compared to the control when incubated with CD112-expressing Huh7 cells, indicating strong binding of NBP3-48298 to CD112. Antibodies were incubated at 5 ug/mL.
Nectin-2/CD112 Antibody (DMC224) - Chimeric

Flow Cytometry: Nectin-2/CD112 Antibody (DMC224) - Chimeric [NBP3-48298]

Nectin-2/Nectin-2/CD112 protein is highly expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with Anti-Nectin-2/Nectin-2/CD112 (DMC224) on HEK293 cells transfected with human Nectin-2/Nectin-2/CD112 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Applications for Nectin-2/CD112 Antibody (DMC224) - Chimeric

Application
Recommended Usage

ELISA

1:5000-10000

Flow Cytometry

1:100

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nectin-2/CD112

Nectin 2 encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Gene Symbol

NECTIN2

Additional Nectin-2/CD112 Products

Product Documents for Nectin-2/CD112 Antibody (DMC224) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nectin-2/CD112 Antibody (DMC224) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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