NEK2 Antibody (5O0A1)

Novus Biologicals | Catalog # NBP3-16641

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O0A1 expressed in HEK293
View all NEK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NEK2 (P51955). MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEK2 Antibody (5O0A1)

Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641]

Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641]

Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for NEK2 Antibody (5O0A1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK2

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Gene Symbol

NEK2

Additional NEK2 Products

Product Documents for NEK2 Antibody (5O0A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NEK2 Antibody (5O0A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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