NEK9 Antibody (1F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00091754-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F6

Format

Azide and BSA Free
View all NEK9 Primary Antibodies »

Product Specifications

Immunogen

NEK9 (AAH09336, 226 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGKGTPLTPPACACSSLQVEVERLQGLVLKCLAEQQKLQQENLQIFTQLQKLNKKLEGGQQVGMHSKGTQTAKEEMEMDPKPDLDSDSWCLLGTDSCRPSL

Reactivity Notes

Human, Mouse, Rat. Other species not tested.

Specificity

NEK9 - NIMA (never in mitosis gene a)- related kinase 9

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NEK9 Antibody (1F6) - Azide and BSA Free

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01]

Immunocytochemistry/ Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01]

Immunocytochemistry/Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of monoclonal antibody to NEK9 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6 Analysis of NEK9 expression in HeLa.
Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in PC-12.
Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in Raw 264.7.
Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01]

Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody (M01), clone 1F6.Lane 1: NEK9 transfected lysate(107.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: NEK9 Antibody (1F6) [H00091754-M01] - Detection limit for recombinant GST tagged NEK9 is approximately 0.03ng/ml as a capture antibody.

Applications for NEK9 Antibody (1F6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEK9

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosomeseparation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phaseprogression

Long Name

Serine/threonine-protein kinase Nek9

Alternate Names

KIAA1995, Nek8, NERCC, Nercc1 kinase

Entrez Gene IDs

91754 (Human)

Gene Symbol

NEK9

OMIM

609798 (Human)

UniProt

AAH09336 (Human)

Additional NEK9 Products

Product Documents for NEK9 Antibody (1F6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NEK9 Antibody (1F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NEK9 Antibody (1F6) - Azide and BSA Free

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Protocols

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FAQs

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