Neurofibromin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29269

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Between 1 and 50

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neurofibromin 1 Antibody - BSA Free

Neurofibromin 1 Antibody

Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-29269] -

Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-29269] -Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NBP3-29269 (lot NBP3-29269-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies. For blotting immunoprecipitated NF1, NBP3-29269 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Neurofibromin 1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Neurofibromin 1

Neurofibromin 1 (NF1) is a suspected negative regulator of the ras signal transduction pathway. Neurofibromin is known to regulate the GTPase HRAS, causing the hydrolyzation and inactivation of GTP. Defects in NF1 lead to Neurofibromatosis disease, Recklinghausen disease or Watson disease. Neurofibromin has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence.

Alternate Names

DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS

Gene Symbol

NF1

Additional Neurofibromin 1 Products

Product Documents for Neurofibromin 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Neurofibromin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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