Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-78763

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
View all NF-H ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed.

Description

This ELISA kit applies to the in vitro quantitative determination of Human pNF-H concentrations in Serum, plasma and other biological fluids.
NFH is phosphorylated on a number of the serines at C-terminal according to the sequence of P12036 in Uniprot.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.26%

Inter-Assay Precision (Precision between assays) CV% 5.01%

Recovery for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

Scientific Data Images for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

ELISA: Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric) [NBP2-78763] -

ELISA: Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric) [NBP2-78763] - Standard Curve Reference

Kit Contents for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NF-H

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)

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