Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NFIC Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 250 and 300 of human Nuclear Factor I/C using the numbering given in entry EAW69324.1 (GeneID 4782).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIC Antibody - BSA Free

Western Blot: NFIC Antibody [NBP1-71879]

Western Blot: NFIC Antibody [NBP1-71879]

Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.
Immunohistochemistry: NFIC Antibody [NBP1-71879]

Immunohistochemistry: NFIC Antibody [NBP1-71879]

Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: NFIC Antibody [NBP1-71879]

Immunohistochemistry: NFIC Antibody [NBP1-71879]

Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Applications for NFIC Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFIC

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-binding transcription factor, CTF5, CTFCCAAT-box-binding transcription factor, MGC20153, NF1-C, NFI, NF-I, NF-I/C, NFI-C, nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, nuclear factor I/C (CCAAT-binding transcription factor), TGGCA-binding protein

Gene Symbol

NFIC

UniProt

EAW69324.1 (Human)

Additional NFIC Products

Product Documents for NFIC Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NFIC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NFIC Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can you tell me if this antibody against NFI-C NBP1-71879 would be suitable for detecting NFI-C in mice.

    A: This Ab should be suitable for detecting NFI-C in mice based on the % homology between the mouse and human protein which is 96.0%. (Our lab recommends use if the % is 85% or above.) As we have not tested this Ab in mice samples you would be eligible for our Innovator's Reward program.

  • Q: I am interested to purchase an NFI-C specific antibody. Has it been tested to see if it cross-reacts with other NFI members?

    A: Unfortunately, we have not tested against other NFI members.

  • Q: Can you tell me if this antibody against NFI-C NBP1-71879 would be suitable for detecting NFI-C in mice.

    A: This Ab should be suitable for detecting NFI-C in mice based on the % homology between the mouse and human protein which is 96.0%. (Our lab recommends use if the % is 85% or above.) As we have not tested this Ab in mice samples you would be eligible for our Innovator's Reward program.

  • Q: I am interested to purchase an NFI-C specific antibody. Has it been tested to see if it cross-reacts with other NFI members?

    A: Unfortunately, we have not tested against other NFI members.

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