NFIX Antibody

Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15038] -

NFIX antibody [N2C2], Internal detects NFIX protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFIX stained by NFIX antibody [N2C2], Internal (NBP2-15038) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Western Blot: NFIX Antibody [NBP2-15038] -

miR-744-5p directly targets NFIX. (A) TargetScan, DIANA-Tarbase, and starBase algorithms were applied to identify potential downstream targets of miR-744-5p. (B) NFIX mRNA expression in OS cells transfected with miR-744-5p mimics. (C) Wild type and mutant binding sequences of miR-744-5p on NFIX. The direct binding was verified by dual-luciferase reporter assay. (D) NFIX protein expression in OS cells transfected with miR-744-5p mimics and miR-744-5p inhibitor. (E) NFIX protein expression in OS cells. ** P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://josr-online.biomedcentral.com/articles/10.1186/s13018-024-04947…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NFIX Antibody [NBP2-15038] -

miR-744-5p directly targets NFIX. (A) TargetScan, DIANA-Tarbase, and starBase algorithms were applied to identify potential downstream targets of miR-744-5p. (B) NFIX mRNA expression in OS cells transfected with miR-744-5p mimics. (C) Wild type and mutant binding sequences of miR-744-5p on NFIX. The direct binding was verified by dual-luciferase reporter assay. (D) NFIX protein expression in OS cells transfected with miR-744-5p mimics and miR-744-5p inhibitor. (E) NFIX protein expression in OS cells. ** P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://josr-online.biomedcentral.com/articles/10.1186/s13018-024-04947…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NFIX Antibody [NBP2-15038] -

NFIX neutralized the effects of miR-744-5p on osteosarcoma cells. (A) Protein expression of NFIX in OS cells transfected with NFIX overexpression vector. (B) Protein expression of NFIX in different groups of OS cells. (C) OS cell viability was assessed by CCK-8 assay. (D) Cell proliferation was visualized by colony formation assay. (E) Flow cytometry assay was performed to determine cell apoptosis rate. (F) The viability of HUVECs was assessed by CCK-8 assay. (G) The effects of miR-744-5p and NFIX on the tube formation of HUVECs were detected by tube formation assay. *P < 0.05; ** P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://josr-online.biomedcentral.com/articles/10.1186/s13018-024-04947…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NFIX Antibody [NBP2-15038] -

NFIX neutralized the effects of miR-744-5p on osteosarcoma cells. (A) Protein expression of NFIX in OS cells transfected with NFIX overexpression vector. (B) Protein expression of NFIX in different groups of OS cells. (C) OS cell viability was assessed by CCK-8 assay. (D) Cell proliferation was visualized by colony formation assay. (E) Flow cytometry assay was performed to determine cell apoptosis rate. (F) The viability of HUVECs was assessed by CCK-8 assay. (G) The effects of miR-744-5p and NFIX on the tube formation of HUVECs were detected by tube formation assay. *P < 0.05; ** P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://josr-online.biomedcentral.com/articles/10.1186/s13018-024-04947…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.