NG2/MCSP Antibody (DMC466) - Chimeric

Novus Biologicals | Catalog # NBP3-48349

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC466
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

NG2/MCSP recombinant protein expressed in mammalian cells (Accession# Q6UVK1). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for NG2/MCSP Antibody (DMC466) - Chimeric

NG2/MCSP Antibody (DMC466) - Chimeric

Flow Cytometry: NG2/MCSP Antibody (DMC466) - Chimeric [NBP3-48349]

NG2/MCSP protein is highly expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with Anti-NG2/MCSP (DMC466) on HEK293 cells transfected with human NG2/MCSP (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Applications for NG2/MCSP Antibody (DMC466) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NG2/MCSP

Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (DMC466) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NG2/MCSP Antibody (DMC466) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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