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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human NGRN fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for NGRN Antibody - BSA Free
Western Blot: NGRN Antibody [NBP3-12633]
Western Blot: NGRN Antibody [NBP3-12633] - Lane A: A549 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:34 kDaImmunoprecipitation: NGRN Antibody [NBP3-12633]
Immunoprecipitation: NGRN Antibody [NBP3-12633] - Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-NGRN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NGRN rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size :34 kDaApplications for NGRN Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NGRN
Long Name
Neugrin
Alternate Names
DSC92, Neugrin
Gene Symbol
NGRN
Additional NGRN Products
Product Documents for NGRN Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NGRN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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