NHP2L1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32732

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Full length human NHP2L1 Recombinant protein.

Localization

Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHP2L1 Antibody - BSA Free

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732]

Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732]

Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732]

Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732]

Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.
NHP2L1 Antibody NHP2L1 Antibody

NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed zebrafish embryos. NHP2L1 antibody [N1C3] dilution: 1:200.

NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed zebrafish embryos. NHP2L1 antibody [N1C3] dilution: 1:200.

Applications for NHP2L1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 26106861). IHC-Wm Assay dependent.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NHP2L1

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

[U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein, 15.5K, FA1, FA-1, High mobility group-like nuclear protein 2 homolog 1, hSNU13, NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae), NHP2-like protein 1, NHPX, non-histone chromosome protein 2 (S. cerevisiae)-like 1, non-histone chromosome protein 2-like 1, OTK27, small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5), SNRNP15-5, SNU13, SNU13 homolog, SPAG12, sperm specific antigen 1, SSFA1, U4/U6.U5 tri-snRNP 15.5 kDa protein

Entrez Gene IDs

4809 (Human); 20826 (Mouse); 300092 (Rat)

Gene Symbol

SNU13

UniProt

Additional NHP2L1 Products

Product Documents for NHP2L1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NHP2L1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for NHP2L1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NHP2L1 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Have you guys tested the NHP2L1 antibodies (ANTI-15.5K ANTIBODY) (cat# NBP1-32732) for IP experiments? Can it be used for whole cell extract 15.5K immunoprecipitation?

    A:

    This is not an application we have tested, and therefore we cannot say for certain whether this application will work. Due to the fact that it does work in WB there is a good chance the antibody will also work in IP. Even though Novus cannot guarantee it, we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If the testing for this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

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