NI 57

Key Product Details

Product Description

NI 57 is a potent and selective BRPF bromodomain inhibitor (K_d values are 31, 108 and 408 nM, for BRPF1B, 2 and 3, respectively, which exhibits >32-fold selectivity for BRPFs over BRD9 and other non-Class IV bromodomains. NI 57 inhibits RANKL-induced differentiation of primary murine bone marrow cells and human primary monocytes into osteoclasts.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the NI 57 probe summary on the SGC website.

External Portal Information

Chemicalprobes.org

is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of NI 57 is reviewed on the chemical probes website.

Product Specifications for NI 57

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for NI 57

The following data is based on the product molecular weight 383.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.61 mL	13.04 mL	26.08 mL
5 mM	0.52 mL	2.61 mL	5.22 mL
10 mM	0.26 mL	1.30 mL	2.61 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Meier Selective targeting of bromodomains of the bromodomain-PHD fingers family impairs osteoclast differentiation. ACS Chem.Biol. 2017 PMID: 28849908
• Igoe Design of a chemical probe for the bromodomain and plant homeodomain finger-containing (BRPF) family of proteins. J.Med.Chem. 2017 PMID: 28714688

Product Documents for NI 57