NIK/MAP3K14 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-56367

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NIK/MAP3K14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 687-703 of human NIK.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIK/MAP3K14 Antibody - Azide and BSA Free

Western Blot: NIK/MAP3K14 Antibody [NB100-56367]

Western Blot: NIK/MAP3K14 Antibody [NB100-56367]

Western Blot: NIK/MAP3K14 Antibody [NB100-56367] - Analysis of NIK/MAP3K14 in (A) untransfected and (B) transfected 293 cells using this antibody.
Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367]

Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367]

Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with NIK/MAP3K14 (NB100-56367) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.

Applications for NIK/MAP3K14 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIK/MAP3K14

NIK (NF-kB-inducing Kinase) expression leads to IkB kinase activation and induces nuclear translocation of NF-kB. It is involved in regulation of apoptosis, tumorigenesis, inflammation and various autoimmune diseases. In PC12 cells it induces differentiation and prevents apoptosis.

Long Name

NF-kappa-beta Inducing Kinase

Alternate Names

FTDCR1B, HSNIK, MAP3K14

Entrez Gene IDs

9020 (Human)

Gene Symbol

MAP3K14

UniProt

Q99558

Additional NIK/MAP3K14 Products

Product Documents for NIK/MAP3K14 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NIK/MAP3K14 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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