NIK/MAP3K14 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-56367

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NIK/MAP3K14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 687-703 of human NIK.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIK/MAP3K14 Antibody - Azide and BSA Free

Western Blot: NIK/MAP3K14 Antibody [NB100-56367]

Western Blot: NIK/MAP3K14 Antibody [NB100-56367]

Western Blot: NIK/MAP3K14 Antibody [NB100-56367] - Analysis of NIK/MAP3K14 in (A) untransfected and (B) transfected 293 cells using this antibody.
Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367]

Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367]

Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with NIK/MAP3K14 (NB100-56367) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.

Applications for NIK/MAP3K14 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1-3 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56367 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIK/MAP3K14

NIK (NF-kB-inducing Kinase) expression leads to IkB kinase activation and induces nuclear translocation of NF-kB. It is involved in regulation of apoptosis, tumorigenesis, inflammation and various autoimmune diseases. In PC12 cells it induces differentiation and prevents apoptosis.

Long Name

NF-kappa-beta Inducing Kinase

Alternate Names

FTDCR1B, HSNIK, MAP3K14

Entrez Gene IDs

9020 (Human)

Gene Symbol

MAP3K14

UniProt

Q99558

Additional NIK/MAP3K14 Products

Product Documents for NIK/MAP3K14 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NIK/MAP3K14 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NIK/MAP3K14 Antibody - Azide and BSA Free (1)

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  • IHQ with NIK/MAP3K14 Antibody
    Name: Aranzazu Pintor
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Kidney tissue and riñon
    Species: Mouse
    Verified Customer | Posted 09/29/2026
    Longitudinal section of the renal cortex from a 3-month-old mouse, paraffin-embedded and sectioned at 3 µm thickness.
    We used the NIK/MAP3K14 Antibody – Azide and BSA Free for immunohistochemistry in mouse kidney tissue at a 1:100 dilution. The samples were obtained from mice treated with cisplatin, with or without a NIK inhibitor, as part of a study investigating NIK-related signaling in kidney injury. The antibody provided consistent and clearly detectable staining under our experimental conditions, with a good signal-to-background ratio. The 1:100 dilution worked well for our protocol and allowed us to identify differences in NIK staining between experimental groups. Tissue morphology was well preserved and the staining pattern was suitable for subsequent histological analysis and comparison between cisplatin-treated and inhibitor-treated samples. Overall, the antibody performed reliably for immunohistochemical detection of NIK/MAP3K14 in mouse kidney tissue and was suitable for our experimental application.
    NIK/MAP3K14 Antibody - Azide and BSA Free NB100-56367

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