NIR Dye s775z, NHS

Tocris Bioscience | Catalog # 7626

Bright and photostable non-aggregating near-IR cyanine dye (Reactive group: NHS ester). Suitable for in vivo imaging
Tocris Bioscience

Key Product Details

Description

Bright and photostable non-aggregating near-IR cyanine dye (Reactive group: NHS ester). Suitable for in vivo imaging

Wavelength

Excitation = 775 nm, Emission = 795 nm
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Product Description

Key information:

NIR Dye s775z, NHS is a near-infrared (NIR) fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for in vivo imaging.

Application: Fluorescence microscopy, in vivo imaging.

Properties and Photophysical Data: When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation and emission maxima (λ) are 775 nm and 795 nm, respectively; quantum yield = 0.09; extinction coefficient = 201,000 M-1cm-1; A280 correction factor = 0.11.

For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Scientific Data Images for NIR Dye s775z, NHS

Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma.

Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma.

HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with NIR Dye s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale: 30 μm. For more information on this work read Schreiber et al (2021) High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567

Application of NIR Dye s775z, NHS in vivo

Application of NIR Dye s775z, NHS in vivo.

Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body. For more information on this work read Gamage et al (2021) Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer. ACS Omega 6 30130 PMID: 34778684

Licensing Information

Sold under license from the University of Notre Dame

Optical Data

NIR Dye s775z, NHS Dye Spectra
Emission Color Near-IR
Brightness
λabs 775 nm
λem 795 nm
Extinction Coefficient (ε) 201000 M-1cm-1
Quantum Yield (φ) 0.09
Closest Laser line 750 nm
Reactive Group NHS ester
Reactivity Primary amines
Correction Factor 280 0.11
Application Fluorescence Microscopy, In Vivo Imaging
Alternative For FNIR-Tag, IRDye® 800CW, UL766

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Product Specifications for NIR Dye s775z, NHS

Molecular Weight

1507.22

Formula

C72H100ClN11O18S2

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2-((1E,3Z,5E)-4-(2,6-bis((4-(2,5,8,11-tetraoxadodecyl)-1H-1,2,3-triazol-1-yl)methyl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-7-((E)-3,3-dimethyl-5-sulfonato-1-(3-(trimethylammonio)propyl)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-(3-(trimethylammonio)propyl)-3H-indol-1-ium-5-sulfonate chloride

InChI Key

BMWUPMKMGLAKDQ-UHFFFAOYSA-M

SMILES

CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)N(CCC[N+](C)(C)C)/C1=C/C=C/C(C3=C(CN4C=C(COCCOCCOCCOC)N=N4)C=C(C(ON(C(CC5)=O)C5=O)=O)C=C3CN6N=NC(COCCOCCOCCOC)=C6)=C/C=C/C7=[N+](CCC[N+](C)(C)C)C8=C(C=C(S(=O)([O-])=O)C=C8)C7(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for NIR Dye s775z, NHS

Certificate of Analysis

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Product Specific Notices for NIR Dye s775z, NHS

For research use only

Citations for NIR Dye s775z, NHS

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