NIR Dye s775z, NHS

Catalog # Availability Size / Price Qty
NIR Dye s775z, NHS | Fluorescent Dyes
3 Images
Description: Bright and photostable non-aggregating near-IR cyanine dye. Reactive group: NHS ester

Chemical Name: 2-((1E,3Z,5E)-4-(2,6-bis((4-(2,5,8,11-tetraoxadodecyl)-1H-1,2,3-triazol-1-yl)methyl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-7-((E)-3,3-dimethyl-5-sulfonato-1-(3-(trimethylammonio)propyl)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-(3-(trimethylammonio)propyl)-3H-indol-1-ium-5-sulfonate chloride

Purity: ≥90%

Product Details

Biological Activity

NIR Dye s775z, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation/emission maxima = 775/795 nm; extinction coefficient = 201000 M-1cm-1; quantum yield = 0.09.

For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Scientific Data

Fluorescence Imaging Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. View Larger

Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with NIR Dye s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale: 30 μm. For more information on this work read Schreiber et al (2021) High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567

In vivo Imaging Application of NIR Dye s775z, NHS in vivo View Larger

Application of NIR Dye s775z, NHS in vivo. Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body. For more information on this work read Gamage et al (2021) Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer. ACS Omega 6 30130 PMID: 34778684

Optical Data for NIR Dye s775z, NHS

NIR Dye s775z, NHS Dye Spectra
Emission Color Near-IR
λabs 775 nm
λem 795 nm
Extinction Coefficient (ε) 201000 M-1cm-1
Quantum Yield (φ) 0.09
Closest Laser line 750 nm
Reactive Group NHS ester
Reactivity Primary amines
Application Fluorescence microscopy, In vivo Imaging
Alternative For FNIR-Tag, IRDye® 800CW, UL766

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the University of Notre Dame

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for NIR Dye s775z, NHS

There are currently no reviews for this product. Be the first to review NIR Dye s775z, NHS and earn rewards!

Have you used NIR Dye s775z, NHS?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.