NIR Dye s775z, NHS New
Chemical Name: 2-((1E,3Z,5E)-4-(2,6-bis((4-(2,5,8,11-tetraoxadodecyl)-1H-1,2,3-triazol-1-yl)methyl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-7-((E)-3,3-dimethyl-5-sulfonato-1-(3-(trimethylammonio)propyl)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-(3-(trimethylammonio)propyl)-3H-indol-1-ium-5-sulfonate chloride
Biological ActivityNIR Dye s775z, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation/emission maxima = 775/795 nm; extinction coefficient = 201000 M-1cm-1; quantum yield = 0.09. For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with NIR Dye s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale: 30 μm. For more information on this work read Schreiber et al (2021) High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567
Application of NIR Dye s775z, NHS in vivo. Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body. For more information on this work read Gamage et al (2021) Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer. ACS Omega 6 30130 PMID: 34778684
Optical Data for NIR Dye s775z, NHS
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