NKX6.1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-82553]

NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0018.jpg

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553]

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553]

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human liver shows no positivity as expected.

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody - BSA Free [NBP1-82553] -

Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. (a) Scheme depicting the three cell populations analysed by immunofluorescence. (b) Proportion of the differentiated hiPSC-cells expressing insulin, glucagon or somatostatin in the three distinct populations analysed, quantified by Imaris software. (c) Proportion of bihormonal cells in the three distinct populations analyzed. (d) Proportion of the differentiated hiPSC-cells expressing PDX1 or NKX6.1 in the three distinct populations analysed. (e) Proportion of insulin + cells coexpressing PDX1 or NKX6.1. (f) High magnification confocal images of cells inside alginate capsules stained for insulin (green), glucagon (red), somatostatin (purple) and DAPI (blue) by whole mount immunofluorescence. (g) Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31942009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody - BSA Free [NBP1-82553] -

Wnt-modulation influences the distribution of mono-hormonal and bi-hormonal S7 cells. (A) Representative schematic drawing and IF analysis for insulin+ (red), glucagon+ (green) cells and insulin+/glucagon+ cells (yellow) of the respective S7 cell populations. (B) Calculations of insulin+ (red, left) and glucagon+ (green, right) cells were done as described in Methods. The y-axis shows the number of insulin+ and glucagon+ cells, respectively. The figure shows standard error of the mean (SEM) values for each of the columns in bar charts. **P < 0.006, ****P < 0.0001 vs. S7 cells with two-tailed t-test. No significant comparisons show no stars. The number of insulin+ cells and glucagon+ cells were normalized to total cell count (dapi+ cells) see Supplementary Figure 2. (C) Overlay of insulin+ and glucagon+ were used to count bi-hormonal cells, in which bi-hormonal (ins+glu+) cells were calculated as a fraction of insulin+ (red bar chart) and glucagon+ (green bar chart) cells, respectively. The figure shows standard error of the mean (SEM) values for each of the columns in bar charts. **P < 0.006, ****P < 0.0001 vs. S7 cells with two-tailed, type two t-test. No significant comparisons show no stars. (D) IF analysis of NKX6.1+ (red), PDX1+ (green) cells and NKX6.1+/PDX1+ cells (yellow). Scale bar upper panel: 25 μm, lower panel: 7.5 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31139151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.