Recombinant Human NME4 His Protein

Name: Recombinant Human NME4 His Protein
Brand: Novus Biologicals
SKU: NBP2-52254
Price: 159 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52254

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE

View all NME4 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-187 of Human NME4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NME4 His Protein

SDS-PAGE: Recombinant Human NME4 His Protein [NBP2-52254]

SDS-Page: NME4 Recombinant Protein [NBP2-52254] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52254

Formulation	20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.2 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NME4

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM]

Alternate Names

EC 2.7.4.6, NDP kinase, mitochondrial, NDPK-D, NM23D, nm23-H4mitochondrial, non-metastatic cells 4, protein expressed in

Gene Symbol

NME4

Additional NME4 Products

Product Documents for Recombinant Human NME4 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human NME4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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