Non-Human Primate CCL19/MIP-3b Cartridge

R&D Systems | Catalog # SPCKB-NP-011294

Simple Plex Non-Human Primate MIP-3b assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by NP (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.35 pg/mL

Assay Range

3.93-6000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Non-Human Primate CCL19/MIP-3b Cartridge Summary

Simple Plex assay for the detection of non-human primate Macrophage Inflammatory Protein 3 beta (MIP-3 beta ) in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CCL19/MIP-3 beta Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant MIP-3 beta. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 37 36
Mean (pg/mL) 36.4 2055 34.8 1881
Standard Deviation 1.57 115 3.51 209
CV% 4.3 5.6 10.1 11.1

Recovery for Non-Human Primate CCL19/MIP-3b Cartridge

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 86 79-101
Heparin Plasma (n=5) 82 78-88
Serum (n=5) 81 76-89

Linearity

Samples containing and/or spiked with high concentrations of MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Non-Human Primate CCL19/MIP-3b Cartridge

Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Alternate Names

ELC, MIP-3 beta, MIP3 beta

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Gene Symbol

CCL19

Additional CCL19/MIP-3 beta Products

Product Documents for Non-Human Primate CCL19/MIP-3b Cartridge

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Certificate of Analysis

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Product Specific Notices for Non-Human Primate CCL19/MIP-3b Cartridge

For research use only

Citations for Non-Human Primate CCL19/MIP-3b Cartridge

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