Non-Human Primate CCL2/MCP-1 Cartridge

Name: Non-Human Primate CCL2/MCP-1 Cartridge
Brand: R&D Systems
SKU: SPCKB-NP-011281
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-NP-011281

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Product Documents

Simple Plex Non-Human Primate MCP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.35 pg/mL

Assay Range

1.52-5780 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Non-Human Primate CCL2/MCP-1 Cartridge Summary

Simple Plex assay for the detection of non-human primate Monocyte Chemotactic Protein 1 (MCP-1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CCL2/JE/MCP-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant MCP-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	18
Mean (pg/mL)	33.2	1746	32.3	1744
Standard Deviation	1.19	124	2.05	136
CV%	3.6	7.1	6.3	7.8

Recovery for Non-Human Primate CCL2/MCP-1 Cartridge

Recovery was not necessary as MCP-1 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of MCP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Non-Human Primate CCL2/MCP-1 Cartridge

Non-Human Primate CCL2/MCP-1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4⁺ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.