Non-Human Primate PD-L1/B7-H1 Cartridge

R&D Systems | Catalog # SPCKB-NP-011465

Simple Plex Non-Human Primate PD-L1/B7-H1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by NP (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.19 pg/mL

Assay Range

0.56-5332 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Non-Human Primate PD-L1/B7-H1 Cartridge Summary

Simple Plex assay for the detection of non-human primate B7 Homolog 1 (B7-H1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all PD-L1/B7-H1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant B7-H1.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 49 48
Mean (pg/mL) 74.9 3685 71.7 3750
Standard Deviation 6.64 225 6.99 253
CV% 8.9 6.1 9.7 6.8

Recovery for Non-Human Primate PD-L1/B7-H1 Cartridge

Recovery was not necessary as B7-H1 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of B7-H1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Non-Human Primate PD-L1/B7-H1 Cartridge

Non-Human Primate PD-L1/B7-H1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 protein binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells. Many tumors overexpress PD-L1 protein making the PD-1:PD-L1 interaction central to several successful cancer immunotherapy approaches.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

Additional PD-L1/B7-H1 Products

Product Documents for Non-Human Primate PD-L1/B7-H1 Cartridge

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Certificate of Analysis

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Product Specific Notices for Non-Human Primate PD-L1/B7-H1 Cartridge

For research use only

Citations for Non-Human Primate PD-L1/B7-H1 Cartridge

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